ExgeneTM Plant SV kit provides a simple and easy method for the small, medium and large scale purification of total DNA from various plant tissues. With EzSepTM filter and column type G, the procedure can be done in just 40 minutes ( mini) , yielding a pure genomic DNA suitable for various downstream appilcations without further manipulation. Up to 100 mg, 400 mg of plant tissue can be processed with ExgeneTM Plant SV mini, Midi and MAXI, respectively. ExgeneTM Plant SV procedure eliminates the need of organic solvent extraction and alcohol precipitation, allowing safe and fast preparation of many samples simultaneously. Purified total DNA can be directly applicable in conventional PCR, real-time PCR, Southern blotting, SNP genotyping, RFLP, AFLP and RAPD. FEATURES & BENEFITS : - Spin or vacuum column format - Stable and consistent DNA extraction from plant cells, tissues and fungi - Instant use : No need of additional materials Perfect removal of second metabolites such as polyphenols and polysaccharides - Simple procedure by the use of EzSepTM filter column - No use of organic solvents - Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions SPECIFICATIONS : - Format : Midi SV column type G with 15 ml collection tubes - Sample size : Up to 400 mg wet ( 100 mg dry) - Preparation time : < 1 hour - Typical yield : 10-150 µ g - Elution volume : 200-600 µ - Storage : Room temperature ( 15-25° C) - Size : 26, 100
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