HyperScript™ Reverse Transcriptase

HyperScript™ Reverse Transcriptase is a new engineered M-MLV Reverse Transcriptase with reduced RNase H activity and increased thermal stability. It can be used to synthesize first-strand cDNA at a temperatures range up to 65° C. So it provides increased specificity, higher yields of cDNA and more full-length product than other reverse transcriptases. It makes that up to 13 kb in length can be synthesized accurately. The amount of starting material can be adjusted from 1 pg to 2 ug of total RNA. cDNA synthesis can be performed using either total RNA or the poly( A) -selected RNA, primed with oligo dT, random primer or a gene specific primer. COMPONENTS : - HyperScript™ Reverse Transcriptase ( 200 U/ • ) : 1 vial ( 50 • ) - 10X RTase reaction buffer : 1 vial ( 1000 • ) - 0.1 M DTT : 1 vial ( 250 • ) - 10 mM dNTP mix : 1 vial ( 250 • ) SPECIFICATIONS : - New MMLV originated Reverse Transcriptase - Thermostable RTase ( up to 65° C) - Enhanced Performance : highly efficient and sensitive transcription of RNA amounts - Full-length cDNA synthesis ( up to 13 kb) - High Reproducibility - Application : RT-PCR, Cloning for protein expression, cDNA library - Storage : -20° C - Size : 50 µ ( 10.000 U)