GeneAll® Exprep™ Plasmid SV provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. Exprep™ Plasmid SV can yield up to 30 μg (mini) of highly purified plasmid DNA and it can be applicable directly for PCR, cloning, automated sequencing, synthesis of labeled hybridization probes and other enzymatic reactions without further manipulation.

Technical Information

Features Plasmid SV mini Plasmid SV Midi
Format Column type Q (mini) with collection tube Column type Q (Midi) with collection tube
Recommended sample volume 5 ml LB 50 ml LB
Maximum sample volume Up to 10 ml LB Up to 100 ml LB
Lysate clearing Centrifugation EzClear™ Filter
Preparation time under 23 minutes under 50 minutes
Maximum loading volume 800 μl 15 ml
Binding capacity 30 μg 300 μg
Recovery rate 85~95% 85~95%
Maximum Elution volume 40 μl 400 μl

Kit Contents

Plasmid SV mini

(Cat. No. 101-150 / 101-102)

Plasmid SV Midi

(Cat. No. 101-226 / 101-201)

  • mini SV column type Q
  • Collection tubes
  • Buffer S1
  • Buffer S2
  • Buffer S3
  • Buffer AW (concentrate)
  • Buffer PW concentrate)
  • Buffer EB
  • RNase A (20 mg/ml)
  • Midi SV column type Q (red ring)
  • EzClear™ Filter column (blue ring)
  • Collection tubes
  • Buffer S1
  • Buffer S2
  • Buffer S3
  • Buffer AW (concentrate)
  • Buffer PW concentrate)
  • Buffer EB
  • RNase A (20 mg/ml)

Alat Laboratorium Kesehatan.