In recent years, interest in small RNA, such as siRNA and miRNA which are related to research of gene regulation, has expanded. There are many commercial kits for total RNA preparation, but most of these are focused on preparation of large RNA longer than 200 nucleotides. Because both siRNA and miRNA are between 15 ~ 30 nucleotides in length, the need of specially optimized kit for small RNA (< 200 nucleotides) is growing rapidly. GeneAll® Hybrid-R™ miRNA is designed for purification of large and small RNA separately from culture cells or animal tissues and co-purification in a single tube is also available by modified protocol.

Technical Information

Features Hybrid-R™ miRNA
Format Column Type B with collection tube
Column Type W with collection tube
Maximum amount of starting samples ~100 mg tissue or ~1 x 107 cells
Maximum loading volume ~700 μl
Minimum elution volume ~30 μl
Maximum Binding capacity ~100 μg

Kit Contents

Hybrid-R™ miRNA (Cat. No. 325-150)
  • RIboExTM
  • Column type B
  • Column type W
  • Collection tubes
  • Buffer SW1
  • Buffer RBW (concentrate)
  • Buffer RNW (concentrate)
  • Nuclease-free water

Alat Laboratorium Kesehatan.