Ribospin™ Plant
GeneAll® Ribospin™ Plant is specially designed for purification of total RNA from various plant tissues such as leaves, stems, roots and picky plant samples. This kit provides the optimized buffer and spin column, which is effective in removing polysaccharides and polyphenolic compounds and isolating intact plant RNA. The procedure of Ribospin™ Plant begins with the disruption of sample in liquid nitrogen using mortar and pestle. The disrupted sample can be lysed in Buffer RPL or REL. Most impurities except RNA in the lysate are eliminated by filtration through EzPure™ Filter and total RNA including a little impurity is bound to the membrane of Column Type W. Survived genomic DNA can be exterminated by on-column DNase I treatment. Whole procedure of Ribospin™ Plant takes only 25 minutes.
| Features | Ribospin™ Plant | ||
|---|---|---|---|
| Format | Column Type W with collection tube EzPureTM Filter with collection tube |
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| Sample size | ~100 mg plant tissue | ||
| Maximum loading volume of EzPureTM Filter | ~600 μl | ||
| Maximum loading volume of Spin column | ~700 μl | ||
| Minimum elution volume | 30 μl | ||
| Binding capacity | ~100 μg | ||
Kit Contents
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Alat Laboratorium Kesehatan.
